Volume 58, Number 3, July - September 2023
|Page(s)||213 - 220|
|Published online||14 September 2023|
Optimization of secure workstation for manipulation of 35S-labelled molecules: a case of in vitro cell metabolic labelling
Institut Necker Enfants Malades, Radioactivity core facility, INSERM U1151, CNRS UMR 8253, Université Paris Cité, Faculté de Santé, 156 rue de Vaugirard, 75015 Paris, France
* Corresponding author: email@example.com
Accepted: 12 April 2023
Pulse-chase and cell metabolic experiments involving 35S-labelled molecules can be detrimental to your health. Suppliers and literature suggest the use of activated charcoal to limit contamination without any precise specification. The aim of this study is to fill in this gap. Here, we perform the usual procedures, which consist in working with the available equipment according to recommendations of radioprotection. We carried out radiotoxicological exams as well as dosimetric follow-ups of the workers. As this procedure leads to internal contamination of the operators and of surface contamination at the workplace, we tested a more secure workstation with collective protection equipment that we validated as limiting contaminations for workers. This study proposes a method to use 35S-labelled molecules in a more secure way, which could be beneficial for the scientific community.
Key words: pulse-chase / Sulphur 35 / internal contamination evaluation / metabolic labelling
© SFRP, 2023
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