Volume 43, Number 5, 200836th annual meeting of the European Radiation Research Society
|Number of page(s)||1|
|Section||Poster Presentation - Biological Dosimetry|
|Published online||03 September 2008|
Variable sensitivity of chromosomes 2, 8 and 14 in human peripheral blood lymphocytes exposed to heavy ions
Joint Institute for Nuclear Research, Joliot-Curie 6, 141980 Dubna, Russian Federation
2 Stockholm University, Department of Genetics Microbiology and Toxicology, Svante Arrhenius väg 16E, room 515, 10691 Stockholm, Sweden
Purpose: To investigate by FISH the distribution of radiation-induced chromosomal aberrations in chromosomes 2, 8 and 14 of 6 donors. Methods: Irradiation of blood from 6 healthy donors (4 male and 2 female) was performed at the accelerators at the Joint Institute for Nuclear Research in Dubna (Russia). Whole blood samples were irradiation with 3 Gy of protons (Phasotron), 3 Gy of 12C-ions (Nuclotron), 4 Gy of 7Li-ions and 3Gy of 11B-ions (Cyclotron U400M). At the position of the samples the beams energy and LET values were following: protons 170 MeV/n and LET ≈ 0.5 keV/µm; 12C 480 MeV/n and LET = 10.6 keV/µm; 7Li 30 MeV/n and LET ≈ 20 keV/µm; 11B 32 MeV/n and LET ≈ 55 keV/µm. Chromosome aberrations were analyzed in prematurely condensed G2-cells harvested after 48 in culture using calyculin A. Chromosomes 2, 8 and 14 were painted in different colors and aberrations scored with the help of an image-analysis system.
Results: Chromosome 2 was generally less sensitive than expected on the basis of its DNA content. A higher than expected frequency of exchanges was found in chromosome 8, however, variable results were obtained for stable and unstable aberrations. The translocation frequency was higher and dicentric frequency was lower then expected. Chromosome 14 revealed the opposite tendency: for stable exchanges we found a lower sensitivity whereas the frequency of unstable exchanges was higher then expected. Generally, chromosome 14 was found to be less radiosensitive than expected. Conclusion: Chromosome 2 appears to be less sensitive to protons as well as to heavy ions than chromosomes 8 and 14. This result is in line with recent results of a study on the sensitivity of chromosomes 2, 8 and 14 to gamma rays.
Key words: chromosome aberrations / high LET
© EDP Sciences, 2008
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