Numéro |
Radioprotection
Volume 43, Numéro 5, 2008
36th annual meeting of the European Radiation Research Society
|
|
---|---|---|
Numéro d'article | 021 | |
Nombre de pages | 1 | |
Section | Poster Presentation - Biological Dosimetry | |
DOI | https://doi.org/10.1051/radiopro:2008662 | |
Publié en ligne | 3 septembre 2008 |
Possible Biomarkers for Ionizing Radiation Exposure in the primary cultured human fibroblasts
Laboratory of Cytogenetics and Tissue Regeneration, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, 139-706 Seoul, Republic of Korea
Many studies have been performed to assess the development and application of potentially useful techniques for biodosimetry. Although chromosome dicentric assay has been used to estimate dose, the technique has limited dose ranges for detection from 0.2 to 4 Gy. In biodosimetry field, specific biomarkers were needed to detect the responses at low dose less than 0.2Gy. We have investigated the altered patterns of genomic DNA methylation in the primary cultured fibroblast cells by low dose (5 and 500mGy) gamma irradiation. Irradiated fibroblast cells were harvested after 4 ~ 72 hrs and methylation status of gene promoter was analyzed by Illumina Methylation Array Chip. Hypermethylation, no-changes, or hypomethylation were detected by the dose and time in the screen. Regions of the stablely hypermethylated and hypomethylated promoter by radiation were reexamined by the methylation-specific polymerase chain reaction (MSP) with selected candidates. Through this analysis, we selected potential biomarkers for biodosimetry study. This is the first report which introduces the application of promoter methylation analysis to biodosimetry field.
Key words: biological dosimetry / methylation / radiation exposure / promoter
© EDP Sciences, 2008
Les statistiques affichées correspondent au cumul d'une part des vues des résumés de l'article et d'autre part des vues et téléchargements de l'article plein-texte (PDF, Full-HTML, ePub... selon les formats disponibles) sur la platefome Vision4Press.
Les statistiques sont disponibles avec un délai de 48 à 96 heures et sont mises à jour quotidiennement en semaine.
Le chargement des statistiques peut être long.