Issue |
Radioprotection
Volume 43, Number 5, 2008
36th annual meeting of the European Radiation Research Society
|
|
---|---|---|
Article Number | 102 | |
Number of page(s) | 1 | |
Section | Poster Presentation - Radiation chemistry, radiation physics and microdosimetry | |
DOI | https://doi.org/10.1051/radiopro:2008651 | |
Published online | 03 September 2008 |
A study of the production of reactive oxygen species by irradiated HEp-2 cells using the dichlorofluorescein a
Institute of Theoretical and Experimental Biophysics, Institutskaya 3, Moscow region, 142290 Pushchino, Russian Federation
The production of reactive oxygen species (ROS) by HEp-2 cells after X-ray irradiation was studied by the dichlorofluorescein assay. 2'7'-Dichlorodihydroluorescein diacetate (50 µM) was added immediately after irradiation, and cells were incubated with the dye at 37oC for 10 min. Then the cells were washed from 2'7'-dichlordihydrofluorescein, and 2'7'-dichlorofluorescein was extracted from cells by treatment with digitonin. The amount of 2'7'-dichlorofluorescein in cells was determined by their fluorescence. Besides, the effect of irradiation on the permeability of cell membrane to 2'7'-dichlorofluorescein was determined. Cells were loaded with 2'7'-dichlorofluorescein by incubation with 20 µM 2'7'-dichlorofluorescein diacetate for 30 min at room temperature. The exit of 2'7'-dichlorofluorescein from control and irradiated cells was determined in a fluorimeter cuvette at 37oC. It was shown that irradiation increased ROS production by HEp-2 cells, but the dose dependence of the effect was distorted. The distortion of the dose dependence was caused by an increase in cell membrane permeability to 2'7'-dichlorofluorescein after irradiation and the exit of 2'7'-dichlorofluorescein from cells.
Key words: reactive oxygen species / dichlorofluorescein / irradiation
© EDP Sciences, 2008
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.