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Table 1
Details of PCR and RFLP procedures for each genotyping assay.
| Gene | SNPs | Primers 5'–3' | Annealing Temp (°C) | Restriction Enzymes | Fragment sizes (bp) |
|---|---|---|---|---|---|
| XRCC1 | Arg194Trp | F: GCC AGG GCC CCT CCT TCA A R:TAC CCT CAG ACC CAC GAG T |
62 | Pvu II | 485(R/R) 396 + 89(W/W) 485 + 396 + 89(R/W) |
| XRCC3 | Thr241Met | F:GGT CGA GTG ACA GTC CAA AC R:TGC AAC GGC TGA GGG TCT T |
60 | Hin1 II | 315 + 140(T/T) 210 + 140 + 105(M/M) 315 + 210 + 140 + 105(T/M) |
| XPD | Lys751Gln | F:CCC CTC TCC CTT TCC TCT GTT R:GCT GCC TTC TCC TGC GAT TA |
60 | Pst I | 245 + 28(K/K) 182 + 63 + 28(Q/Q) 245 + 182 + 63 + 28(K/Q) |
| MSH3 | Ala1036Thr | F:TCT AAC AGG CAA GTA GGA AC R:TAG CCA CAT TTA ATC CAT AAC |
52 | Hha I | 138 + 87(A/A) 225(T/T) 225 + 138 + 87(A/T) |
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