Possible Biomarkers for Ionizing Radiation Exposure in the primary cultured human fibroblasts

Free access article

Issue		Radioprotection Volume 43, Number 5, 2008 36th annual meeting of the European Radiation Research Society


Article Number		021
Number of page(s)		1
Section		Poster Presentation - Biological Dosimetry
DOI		10.1051/radiopro:2008662
Published online		03 September 2008

Radioprotection 2008, Vol. 43, n° 5
DOI: 10.1051/radiopro:2008662

Possible Biomarkers for Ionizing Radiation Exposure in the primary cultured human fibroblasts

H.J. Yun, Y.Y. Kwon, S.Y. Kim, E.J. Kim and C.-M. Kang

Laboratory of Cytogenetics and Tissue Regeneration, Korea Institute of Radiological & Medical Sciences, 215-4 Gongneung-dong, Nowon-gu, 139-706 Seoul, Republic of Korea

Published online: 4 September 2008

Abstract
Many studies have been performed to assess the development and application of potentially useful techniques for biodosimetry. Although chromosome dicentric assay has been used to estimate dose, the technique has limited dose ranges for detection from 0.2 to 4 Gy. In biodosimetry field, specific biomarkers were needed to detect the responses at low dose less than 0.2Gy. We have investigated the altered patterns of genomic DNA methylation in the primary cultured fibroblast cells by low dose (5 and 500mGy) gamma irradiation. Irradiated fibroblast cells were harvested after 4 ~ 72 hrs and methylation status of gene promoter was analyzed by Illumina Methylation Array Chip. Hypermethylation, no-changes, or hypomethylation were detected by the dose and time in the screen. Regions of the stablely hypermethylated and hypomethylated promoter by radiation were reexamined by the methylation-specific polymerase chain reaction (MSP) with selected candidates. Through this analysis, we selected potential biomarkers for biodosimetry study. This is the first report which introduces the application of promoter methylation analysis to biodosimetry field.

What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.

If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.